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Liquid Chromatography (HPLC)

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High-performance liquid chromatography or high-pressure liquid chromatography as it is sometimes referred to, is most commonly called HPLC. This highly versatile chromatographic technique has applications across many industries and is one of our specialties at Analytical Instrument Resource.

Analytical Instrument Resource buys and sells High-performance Liquid Chromatographs (HPLC) products from a variety of manufactures Hewlett Packard or Agilent Technologies, Shimadzu and Thermo Scientific. The majority of our HPLC systems are the Agilent Technologies 1100 and 1200 series.

High Performance Liquid Chromatography (HPLC) is used in a wide variety of industries.  Some of the most common are pharmaceutical, drug, environmental, food, and forensic laboratories.

HPLC is an analytical technique typically used to analyze UV/Visible and Fluorescence absorbing organic compounds.  This analysis is performed by pumping solvents at very high pressure through a column with a particle bed of a specific pore size which is coated with an organic phase that attracts different compounds according to polarity, size, or both.  In most cases compounds are removed from the column bed by an increasing polar solvent concentration.  Each compound elutes from the column by its specific compound attributes such as polarity and size.  Each organic compound exits the column at a specific time (retention time).  As it elutes a detector measures its absorbance at a specific wavelength.  The response is recorded and compared to a known concentration. This allows the user to determine how much of a specific compound is in each sample.

One of the major advantages of HPLC is its ability to analyze heat labile compounds that cannot be done by Gas Chromatography or Gas Chromatography/Mass Spectrometry.  Also, HPLC is very sensitive and can achieve very low detection limits.  HPLC can also measure compounds in the high percent range.  This makes it very versatile in many industries.

The list of products in this category is of common systems that we produce and support on a routine basis and are meant to be examples of systems that we can build from our typical inventory.



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